Electrophoresis of Mucosal Homogenates

The pepsinogens of human gastric mucosa have been the subject of several studies using electrophoretic and chromatographic techniques (Uriel, 1960; Seijffers, Segal, and Miller, 1963; Tang and Tang, 1963; Kushner, Rapp, and Burtin, 1964; Rapp, Aronson, Burtin, and Grabar, 1964; Hanley, Boyer, and Naughton, 1966; Taylor, 1968; Samloff, 1969); it has been shown that gel electrophoresis is superior to other fractionation procedures (Taylor, 1968) but the findings have been divergent. The present study reinvestigates this topic using the collective experience of other workers (Uriel, 1960; Seijffers et al, 1963; Tang and Tang, 1963; Kushner et al, 1964; Rapp et al, 1964; Hanley et al, 1966; Taylor, 1968; Samloff, 1969), defines the pepsinogen pattern of human gastric mucosa, explains the divergent findings ofearlier investigators, investigates the role of gastric disease in thedetermination ofthe pepsinogen pattern, describes a satisfactory column chromatographic method of pepsinogen fractionation, and relates the mucosal pepsinogen pattern to the pepsin patterns of human gastric juice.